Study of Mozzarella cheese behavior during refrigerated storage

Thamara Evangelista Silva, Thamiris Evangelista Silva, Lismaíra Gonçalves Caixeta Garcia, Priscila Alonso dos Santos

Abstract


This study was carried out to evaluate the behavior of high moisture Mozzarella cheese during 60 days of storage at 7° C in BOD incubator for microbiological quality, physicochemical composition, functional properties, and texture profile. The cheeses were purchased in a dairy industry from the city of Rio Verde, GO. The study was performed for 60 days at 20 days intervals between analysis. In order to evaluate if the changes occurred during storage time were significant, the analysis of variance was performed, and the means of the different storage days were compared by the Tukey test at 95% confidence. The results indicated hygienic-sanitary quality in the production of cheeses. The physicochemical composition was influenced by time only in the pH values and protein content; however, they were in accordance with studies already performed. The functional properties of the cheeses were influenced by the storage time, only the free oil content remained stable. The color analysis of the cheeses showed values tending to yellow coloration throughout the storage period. As the primary and secondary proteolysis increased, there was also an increase in the melting capacity and changes in the texture parameters. Mozzarella cheese was suitable for consumption with a storage period of 60 days at 7°C, and the changes that have occurred are acceptable and required because this cheese is commonly used in pizzas.

Keywords


psychrotrophic microorganisms, proteolysis, shelf life.

References


ABNT (ASSOCIAÇÃO BRASILEIRA DE NORMAS TÉCNICAS). Bactérias coliformes totais, coliformes fecais e Escherichia coli em alimentos: determinação do número mais provável (NMP): MB-3463. Rio de Janeiro: ABNT, 1991, 7p.

AOAC (ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS). Official methods of analysis of the AOAC International. 19th edition. Washington: AOAC, 2012.

APHA (AMERICAN PUBLIC HEALTH ASSOCIATION). Compendium of methods for the microbiological examination of foods. Washington: APHA, 2001. 676p.

AYYASH, M. M.; SHAH, N. P. The effect of substitution of NaCl with KCl on chemical composition and functional properties of low-moisture Mozzarella cheese. Journal of Dairy Science, v. 94, n. 8, p. 3761-3768, 2011.

BARUZZI, F. et al. Occurrence of non-lactic acid bacteria populations involved in protein hydrolysis of cold-stored high moisture Mozzarella cheese. Food Microbiology, v. 30, n. 1, p. 37-44, 2012.

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Portaria nº 146, de 07 de março de 1996. Aprova o Regulamento Técnico de Identidade e Qualidade dos Queijos. Diário Oficial da República Federativa do Brasil, Brasília, 11 de março de 1996.

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Portaria nº 364, de 04 de setembro de 1997. Aprova o Regulamento Técnico para Fixação de Identidade e Qualidade de Queijo Mozzarella (Muzzarella ou Mussarela). Diário Oficial da República Federativa do Brasil, Brasília, 04 de setembro de 1997.

BRASIL. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. Instrução Normativa nº 68, de 12 de 2006. Oficializa os Métodos Analíticos Oficiais Físico-Químicos, para Controle de Leite e Produtos Lácteos, Diário Oficial da República Federativa do Brasil, Brasília, 14 de dezembro de 2006.

BRASIL. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. Resolução RDC nº 12, de 2 de janeiro de 2001. Aprova o Regulamento técnico sobre os padrões microbiológicos para alimentos. Diário Oficial da República Federativa do Brasil, Brasília, 10 de janeiro de 2001.

CANTONI, C. et al. Colorazioni anomale e rigonfiamento di formaggi fusi e mozzarelle. Industrie Alimentari, v. 45, p. 276-281, 2006.

CHIESA, M. O. et al. Avaliação da composição química, proteólise e propriedades funcionais do queijo Mussarela comercial com teor reduzido de gordura. Revista do Instituto de Laticínios Cândido Tostes, v. 66, n. 381, p. 28-33, 2011.

FOX, P. F. Proteolysis during cheese manufacture and ripening. Journal of Dairy Science, v. 72, n. 6, p. 1379-1400, 1989.

GARCIA, E. F. et al. Development and quality of a Brazilian semi-hard goat cheese (coalho) with added probiotic lactic acid bacteria. International Journal of Food Science and Nutrition, v. 63, n. 8, p. 947-956, 2012.

GRANATO, D.; MASSON, M. L. Instrumental color and sensory acceptance of soy-based emulsions: a response surface approach. Ciência e Tecnologia de Alimentos, v. 30, n. 4, p. 1090-1096, 2010.

HASSAN, A. N.; FRANK, J. F. Starter cultures and their use. In: MARTH, E. H.; STEELE, J. L. Applied Dairy Microbiology. New York: Marcel Decker, 2001.

JONGHE, V. et al. Influence of storage conditions on the growth of Pseudomonas species in refrigerated raw milk. Applied and Environmental Microbiology, v. 77, n. 2, p. 460-470, 2011.

KHETRA, Y. et al. Storage changes in low sodium-processed Mozzarella cheese prepared using potassium-based emulsifying salts. Dairy Science & Technology, v. 95, n. 5, p. 639-649, 2015.

KINDSTEDT, P. S.; FOX, P. F. Effect of manufacturing factors, composition and proteolysis on the functional characteristics of mozzarella cheese. Critical Reviews in Food Science and Nutrition, v. 33, n. 2, p. 167-187, 1993.

KINDSTEDT, P. S.; FOX, P. F. Modified Gerber test for free oil in melted Mozzarella cheese. Journal of Food Science, v. 56, n. 4, p. 1115-1116, 1991.

KOSIKOWSKI, F.; MISTRY, V. V. Cheese and fermented milk foods. Michigan: Edwards Brothers, 1997. 323p.

MARINHEIRO, M. F. et al. Qualidade microbiológica de queijo Mussarela em peça e fatiado. Semina: Ciências Agrárias, v. 36, n. 3, p. 1329-1334, 2015.

MARSHALL, R. T. Standard Methods for the Examination of Dairy Products. 16th ed. APHA: Washington, D.C. 1992.

OLIVARES, M. L. et al. Effect of protective atmospheres on physicochemical, microbiological and rheological characteristics of sliced Mozzarella cheese. LWT - Food Science and Technology, v. 47, n. 2, p. 465-470, 2012.

OLIVEIRA, M. B. Avaliação de queijos ricota comercializados em Goiânia-GO e queijos processados com diferentes concentrações de leite e adicionados de proteínas de soja e cálcio. 2012. 109 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Goiás, 2012.

PAULA, J. C. J. de. et al. Princípios básicos de fabricação de queijo: do histórico à salga. Revista do Instituto de Laticínios Cândido Tostes, v. 64, n. 367/368, p. 19-25, 2009.

PERRY, K. S. P. Queijos: aspectos químicos, bioquímicos e microbiológicos. Química Nova, v. 27, n. 2, p. 293-300, 2004.

PIAZZON-GOMES, J. et al. Queijo tipo minas frescal com derivados de soja: características físicas, químicas e sensoriais. Ciência e Tecnologia de Alimentos, v. 30, supl. 1, p. 77-85, 2010.

PINTO, C. J. O. et al. Qualidade microbiológica de leite cru refrigerado e isolamento de bactérias psicrotróficas proteolíticas. Ciência e Tecnologia de Alimentos, v. 26, n. 3, p. 645-651, 2006.

R Development Core Team. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing, 2011. Disponível em: http://www.R-project.org Acesso em: 21 set 2018.

REN, D. et al. The effects of k-casein polymorphism on the texture and functional properties of mozzarella cheese. International Dairy Journal, v. 31, n. 2, p. 65-69, 2013.

RICCIARDI, A. et al. Evolution of microbial counts and chemical and physico-chemical parameters in high-moisture Mozzarella cheese during refrigerated storage. LWT - Food Science and Technology, v. 63, n. 2, p. 821-827, 2015.

ROIG, S. M. et al. Avaliação da proteólise e do derretimento do queijo Prato obtido por ultrafiltração. Ciência e Tecnologia de Alimentos, v. 23, supl., p. 177-182, 2003.

ROWNEY, M. K. et al. Salt-induced structural changes in 1-day old Mozzarella cheese and the impact upon free oil formation. International Dairy Journal, v. 14, n. 9, p. 809-816, 2004.

SANTOS, P. A. Avaliação do leite cru refrigerado produzido na região sudoeste do estado de Goiás estocado por diferentes períodos. 2008. 61 p. Tese (Doutorado em Ciência Animal) – Universidade Federal de Goiás, 2008.

STEELE, J. et al. Perspectives on the contribution of lactic acid bacteria to cheese flavor development. Current Opinion in Biotechnology, v. 24, n. 2, p. 135-141, 2013.

UBALDO, J. C. S. R. et al. Bioactive amines in Mozzarella cheese from milk with varying somatic cell counts. Food Chemistry, v. 178, n. 1, p. 229-235, 2015.

WADHWANI, R. et al. Improvement in melting and baking properties of low-fat Mozzarella cheese. Journal of Dairy Science, v. 94, n. 4, p. 1713-1723, 2011.

WOLF, I. V. et al. Study of the chemical composition, proteolysis, lipolysis and volatile compounds profile of commercial Reggianito Argentino cheese: Characterization of Reggianito Argentino cheese. Food Research International, v. 43, n. 4, p. 1204-1211, 2010.

YAZICI, F.; AKBULUT, C. Impact of whey pH at drainage on the physicochemical, sensory, and functional properties of Mozzarella cheese made from buffalo milk. Journal Agricultural and Food Chemistry, v. 55, n. 24, p. 9993-10000, 2007.

ZAMORA, A. et al. Effect of ultrahigh pressure homogenization of Milk on the texture and water-typology of a starter-free fresh cheese. Innovative Food Science and Emerging Technologies, v. 12, n. 4, p. 484-490, 2011.

ZHONG, T. et al. Investigation of different coating application methods on the performance of edible coatings on Mozzarella cheese. LWT - Food Science and Technology, v. 56, n. 1, p. 1-8, 2014.

ZISU, B.; SHAH, N. P. Textural and functional changes in low-fat Mozzarella cheeses in relation to proteolysis and microstructure as influenced by the use of fat replacers, pre-acidification and EPS starter. International Dairy Journal, v. 15, n. 6-9, p. 957-972, 2005.




DOI: https://doi.org/10.14295/2238-6416.v74i2.754

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