Nisin reduces the Staphylococcus aureus count without changing the characteristics of artisanal Minas cheese from Araxá
Abstract
Traditional Araxá cheese is produced with raw milk, which represents a risk to consumers’ health. The action of nisin (100 IU/g and 400 IU/g) against Staphylococcus aureus during the ripening of Artisanal Minas Cheeses from Araxá was evaluated, and nisin reduced the S. aureus count to less than 1 log CFU/g from the beginning to the end of ripening. Nisin slightly increased the pH and moisture content and decreased the fat content of Artisanal Minas Cheese from Araxá; however, with the lower bacteriocin concentration, these changes did not alter the ripening indexes, color, or texture parameters of the cheese, which changed only as a function of ripening. Nisin has the potential to limit the S. aureus growth in Artisanal Minas Cheese from Araxá, without changing the essential characteristics of this artisanal product.
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ANTONIOS, Z. et al. Prevalence, genetic diversity and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bulk tank milk from Greek traditional ovine farms. Small Ruminant Research, v. 125, p. 120–126, 2015. DOI: 10.1016/j.smallrumres.2015.02.009.
ARCURI, E. F. et al. Determination of cheese origin by using 16S rDNA fingerprinting of bacteria communities by PCR e DGGE: Preliminary application to traditional Minas cheese. Food Control, v. 30, n. 1, p. 1–6, 2013. DOI: 10.1016/j.foodcont.2012.07.007.
BRASIL. MINISTÉRIO DA AGRICULTURA, PECUÁRIA E ABASTECIMENTO. SECRETARIA DE DEFESA AGROPECUÁRIA. Instrução Normativa nº 68. Oficializa os Métodos Analíticos Oficiais Físico-Químicos, para Controle de Leite e Produtos Lácteos. Brasília. Diário Oficial da União. 12 de dezembro de 2006.
BRASIL. MINISTÉRIO DA AGRICULTURA, PECUÁRIA E ABASTECIMENTO. SECRETARIA DE DEFESA AGROPECUÁRIA. Portaria nº 146. Aprova os Regulamentos Técnicos de Identidade e Qualidade dos Produtos Lácteos. Diário Oficial da União. 7 de março de 1996.
CARDOSO, V. M. et al. The influence of seasons and ripening time on yeast communities of a traditional Brazilian cheese. Food Research International, v. 69, p. 331–340, 2015. DOI: 10.1016/j.foodres.2014.12.040.
CARMO, L. S. et al. Food poisoning due to enterotoxigenic strains of Staphylococcus present in Minas cheese and raw milk in Brazil. Food Microbiology, v. 19, n. 1, p. 9–14, 2002. DOI: 10.1006/fmic.2001.0444.
CARVALHO, B. D. S. et al. Physico-chemical and rheological properties of Prato cheese during ripening. African Journal of Biotechnology, v. 14, n. 24, p. 2028–2034, 2015. DOI: 10.5897/ajb2015.14639.
CERUTI, R. et al. Effect of increased initial ripening temperature on the sensory characteristics of Reggianito cheese. International Journal of Dairy Technology, v. 67, p. 539–546, 2014. DOI: 10.1016/j.fm.2012.06.001.
COKAL, Y. et al. Presence of L. monocytogenes and some bacterial pathogens in two Turkish traditional foods, Mihalic cheese and Hosmerim dessert. Food Control, v. 26, p. 337–340, 2012. DOI: 10.1016/j.foodcont.2012.01.058.
DIEZHANDINO, I. et al. Microbiological, physico-chemical and proteolytic changes in a Spanish blue cheese during ripening (Valdeón cheese). Food Chemistry, v. 168, p. 134–141, 2015. DOI: 10.1016/j.foodchem.2014.07.039.
EL GALIOU, O. et al. Chemical and microbiological characteristics of traditional homemade fresh goat cheeses from Northern Morocco. Small Ruminant Research, v. 129, p. 108–113, 2015. DOI: 10.1016/j.smallrumres.2015.06.005.
ERCAN, D. et al. Physicochemical, textural, volatile, and sensory profiles of traditional Sepet cheese. Journal of Dairy Science, v. 94, p. 4300–4312, 2014. DOI: 10.3168/jds.2010-3941.
FELICIO, B.A. et al. Effects of nisin on Staphylococcus aureus count and physicochemical properties of Minas Frescal cheese. Journal of Dairy Science, v. 98, p. 4364–4369, 2015. DOI: 10.3168/jds.2015-9520.
FLÓREZ, A. B.; MAYO, B. Diversity and dynamics of antibiotic-resistant bacteria in cheese as determined by PCR denaturing gradient gel electrophoresis. International Journal of Food Microbiology, v. 214, p. 63–69, 2015. DOI: 10.1016/j.ijfoodmicro.2015.07.027.
GADOTTI, C.; NELSON, L.; DIEZ-GONZALEZ, F. Inhibitory effect of combinations of caprylic acid and nisin on Listeria monocytogenes in queso fresco. Food Microbiology, v. 39, p. 1–6, 2014. DOI: 10.1016/j.fm.2013.10.007.
GAO, P. et al. A non-protein nitrogen index for discriminating raw milk protein adulteration via the Kjeldahl method. Analytical Methods, v. 7, n. 21, p. 9166-9170, 2015. DOI: 10.1039/c5ay01422k.
GARDE, S. et al. Fast induction of nisin resistance in Streptococcus thermophilus INIA 463 during growth in milk. International Journal of Food Microbiology, v. 96, p.165–72, 2004. DOI: 10.1016/j.ijfoodmicro.2004.03.023.
GÓMEZ-TORRES, N. et al. Impact of Clostridium spp. on cheese characteristics: microbiology, color, formation of volatile compounds and off-flavors. Food Control, v. 56, p. 186–194, 2015. DOI: 10.1016/j.foodcont.2015.03.025.
HORT, J.; LE GRYS, G. Developments in the textural and rheological properties of UK Cheddar cheese during ripening. International Dairy Journal, v. 11, p. 475–481, 2001.
JAMALI, H. et al. Prevalence and antimicrobial resistance of Staphylococcus aureus isolated from raw milk and dairy products. Food Control, v. 54, p. 383–388, 2015. DOI: 10.1016/j.foodcont.2015.02.013.
KADIROGLU, P.; KOREL, F.; CEYLAN, C. Quantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR. Journal of Microbiological Methods, v. 105, p. 92–97, 2014. DOI: 10.1016/j.mimet.2014.06.022.
LIMA, C. D. L. C. et al. Microbiological, physical-chemical and sensory evaluation of a traditional Brazilian cheese during the ripening process. World Journal of Microbiology and Biotechnology, v. 24, n. 11, p. 2389–2395, 2008. DOI: 10.1007/s11274-008-9751-1.
LIMA, C. D. L. C. et al. Bactérias do ácido láctico e leveduras associadas com o queijo-de-minas artesanal produzido na região da Serra do Salitre, Minas Gerais. Arquivos Brasileiros de Medicina Veterinária e Zootecnia, v. 61, p. 266–272, 2009.
MALHEIROS, P. D. S. et al. Effect of liposome-encapsulated nisin and bacteriocin-like substance P34 on Listeria monocytogenes growth in Minas Frescal cheese. International Journal of Food Microbiology, v. 156, n. 3, p. 272–277, 2012. DOI: 10.1016/j.ijfoodmicro.2012.04.004.
MARTINS, J. M. et al. Determining the minimum ripening time of artisanal Minas cheese, a traditional Brazilian cheese. Brazilian Journal of Microbiology, v. 46, n.1, p. 219-230, 2015. DOI: 10.1590/S1517-838246120131003.
MATA, G. M. S. C.; VANETTI, M. C. D. Comparison of conventional and rapid methods for Salmonella detection in Artisanal Minas Cheese. Journal of Food Research, v. 1, n. 3, p. 178–183, 2012. DOI: 10.5539/jfr.v1n3p178.
McSWEENEY, P. L. H. Biochemistry of cheese ripening. International Journal of Dairy Technology, v. 57, p. 127–144, 2004.
MONTEL, M. et al. Traditional cheeses: rich and diverse microbiota with associated benefits. International Journal of Food Microbiology, v. 177, p. 136–154, 2014. DOI: 10.1016/j.ijfoodmicro.2014.02.019.
PINTO, M. S. et al. The effects of nisin on Staphylococcus aureus count and the physicochemical properties of Traditional Minas Serro cheese. International Dairy Journal, v. 21, p. 90–96, 2011. DOI: 10.1016/j.idairyj.2010.08.001.
PINTO, M. S. et al. Survival of Listeria innocua in Minas Traditional Serro cheese during ripening. Food Control, v. 20, p. 1167–1170, 2009. DOI: 10.1016/j.foodcont.2009.02.007.
POLI, A. et al. Detection of Staphylococcus aureus and enterotoxin genotype diversity in Monte Veronese, a Protected Designation of Origin Italian cheese. Letters in Applied Microbiology, v. 45, n. 5, p. 529–34, 2007. DOI: 10.1111/j.1472-765X.2007.02224.x.
TONG, Z.; NI, L.; LING, J. Antibacterial peptide nisin: a potential role in the inhibition of oral pathogenic bacteria. Peptides, v. 60, p. 32–40, 2014. DOI: 10.1016/j.peptides.2014.07.020.
TUDOREANU, L.; DUMITREAN, L. Texture profile analysis of some Romanian Pasta Filata cheese varieties (Caciocavallo cheese). Bulletin UASVM Agriculture, v. 66, n. 2, p. 512–517, 2009.
YERLIKAYA, O.; KARAGOZLU, C. Effects of ripening period on textural and sensory properties of Capper cheeses. Journal of Animal and Veterinary Advances, v. 10, n. 9, p. 1171–1176, 2011. DOI: 10.3923/javaa.2011.1171.1176.
ZHAO, X. et al. In Vitro synergy of nisin and coenzyme Q0 against Staphylococcus aureus. Food Control, v. 46, p. 368–373, 2014. DOI: 10.1016/j.foodcont.2014.05.051.
ZHAO, X. et al. Analysis of the gene expression pro fi le of Staphylococcus aureus treated with nisin. Food Control, v. 59, p. 499–506, 2016. DOI: 10.1016/j.foodcont.2015.06.018.
DOI: https://doi.org/10.14295/2238-6416.v74i1.729
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